Figure 6.

Down-regulation of FLIP with CDDO. (A) CLL cells were CD40 activated and replated in media alone for an additional 24 h with (−) or without (+) 1 μM CDDO. Cells were then harvested for immunoblot analysis of FLIP and (B) FADD or (C) CD95 apoptosis assays. Data shown are the mean CD95-mediated apoptosis of four patient samples up to 72 h after CD40 activation in the absence (open bar) or presence (black bar) of 1 μM CDDO treatment, ±SD. In one sample (gray bar), sensitivity to CD95-mediated apoptosis was tested at day 5 after CD40 activation to confirm that CDDO-treated CLL cells still acquired sensitivity to CD95. Apoptosis of Jurkat cells demonstrates activity of CHO-FasL (hatched bars).