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. 2002 Mar 12;99(6):3854–3859. doi: 10.1073/pnas.022604399

Figure 8.

Figure 8

Immunoprecipitation of DISC. CLL cells were CD40-activated and harvested at days 1 and 5 for DISC immunoprecipitation. BJAB- or CD40-activated CLL cells were either untreated or stimulated with 2 μg/ml of APO-1 mAb for 15 min before the preparation of cell lysates. CD95 DISC was immunoprecipitated by using protein A-Sepharose beads (+). Unstimulated controls were lysed before immunoprecipitation with APO-1 and protein A-Sepharose beads (−). Immunoprecipitates were then subjected to immunoblot analysis with anti-FLIP mAb (Upper) or anti-FADD (Lower). A431- and CD40-activated CLL cell lysates were loaded as controls for the migration position of FADD and FLIP.