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. 2002 Mar 19;99(6):3890–3895. doi: 10.1073/pnas.062047499

Figure 1.

Figure 1

Loss of E2F1 has minor effects on expression of known target genes. (A) Reverse transcription–PCR was used to analyze the levels of dhfr, TK, cyclin E, and Rb mRNA in RNA prepared from the livers of C3H/HeJ and C57BL/6J wt vs. E2F1 nullizygous newborn mice. (B) Chromatin immunoprecipitations were performed from C57BL/6J wt and nullizygous E2F1 embryos using antibodies to E2F1 to -6, and a no antibody control. The chromatin was then analyzed by PCR using primers specific for the dhfr, TK, cyclin E, and Rb promoters. For this experiment, as well as those shown in Figs. 3 and 4, the amount of chromatin used in the PCR reaction of the input lane represents 0.4% of the starting material, and the amount of sample used in the PCR reaction of the immunoprecipitated lane represents 6.7% of the precipitated chromatin. Therefore, approximately equal signals in the sample and input lanes suggest that about 6% of the starting material was precipitated by the antibody.