Figure 3.

The identification of E2F1-specific target promoters. For both A and B, the controls and amounts of chromatin used are as described in Fig. 1; the chromatin was analyzed by using primers shown in C. (A) A chromatin immunoprecipitation experiment was performed from liver isolated from adult C3H/HeJ wt or E2F1 nullizygous mice using antibodies to the E2F family members, as well as a no antibody control. (B) A chromatin immunoprecipitation experiment was performed from liver isolated from adult C3H/HeJ wt and E2F1 nullizygous mice using antibodies to C/EBPα and C/EBPβ, as well as a no antibody control. (C) Shown is a schematic of the E2F1-specific promoters and the approximate location of the primers (arrows) used in the chromatin immunoprecipitation experiments. The numbers are relative to +1 being the 5′ end of the mRNA (indicated by a bent arrow).