Table 4.
Primer Sequences |
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Variant | Forward | Reverse | Annealing Temperature(°C) | Restriction Enzyme |
rs11538141 | CAG AGT CGC CAT GCA GAT CC | CCC GAA GGG CAC CAC GCA CT | 58 | MnlI |
rs2736911 | GCA CCT TTG TCA CCA CAT TA | GCC TGA TCA TCT GCA TTT CT | 54 | DraIII |
rs10490923 | GCA CCT TTG TCA CCA CAT TA | GCC TGA TCA TCT GCA TTT CT | 54 | HhaI |
rs10490924 | GCA CCT TTG TCA CCA CAT TA | GCC TGA TCA TCT GCA TTT CT | 54 | PvuII |
rs1803403 | TGC TGT CCC TTT GTT GTC TC | AGA CAC AGA CAC GCA TCC TG | 55 | NA |
rs12258692 (and rs4405249) | GCC AGG AAA AGG AAC CTC | GCC AGG CAT CAA GTC AGA | 54 | NA |
Note.— NA = not applicable.