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. 2005 Jul 26;77(3):389–407. doi: 10.1086/444437

Table 4.

Primers, Annealing Conditions, and Restriction Endonucleases Used for Genotype Data Collection[Note]

Primer Sequences
Variant Forward Reverse Annealing Temperature(°C) Restriction Enzyme
rs11538141 CAG AGT CGC CAT GCA GAT CC CCC GAA GGG CAC CAC GCA CT 58 MnlI
rs2736911 GCA CCT TTG TCA CCA CAT TA GCC TGA TCA TCT GCA TTT CT 54 DraIII
rs10490923 GCA CCT TTG TCA CCA CAT TA GCC TGA TCA TCT GCA TTT CT 54 HhaI
rs10490924 GCA CCT TTG TCA CCA CAT TA GCC TGA TCA TCT GCA TTT CT 54 PvuII
rs1803403 TGC TGT CCC TTT GTT GTC TC AGA CAC AGA CAC GCA TCC TG 55 NA
rs12258692 (and rs4405249) GCC AGG AAA AGG AAC CTC GCC AGG CAT CAA GTC AGA 54 NA

Note.— NA = not applicable.