Figure 3.

BPI functionally regulates epithelial endotoxin responses. (A) Transcriptional induction of ICAM-1 was examined in KB cells in response to indicated concentrations of endotoxin (LPS) in the presence of NHS (5% vol/vol) or IL-1 (10 ng/ml) for 8 h. Total RNA was isolated and used to assess ICAM-1 transcripts by RT-PCR. β actin transcript was used as an internal standard. (B) Endotoxin-induced ICAM-1 expression was examined in the presence polyclonal anti-BPI (+) or in the presence of NGS (−). β actin transcript was used as an internal standard. (C) KB cells were preexposed to ATLa (1 μM, 8 h), and cell surface ELISA was used to screen ICAM-1 protein induction by endotoxin/5% NHS (additional 24 h) in the presence of anti-BPI or control NGS. (*, significantly different from NGS; P < 0.025)