Figure 3.

Fast decay and run-down of AcCho currents elicited by mutα7-GFP receptors. AcCho currents evoked in oocytes expressing mutα7 (A) or mutα7-GFP (C) receptors. Note the faster AcCho-current decay compared with A. (B) Superimposed traces of AcCho currents in two other oocytes. AcCho-current amplitudes: 7.3 μA (mutα7); 1.4 μA (mutα7-GFP). Holding potential, −100 mV. (D) AcCho-current amplitudes of consecutive responses in oocytes injected with mutα7 or mutα7-GFP subunit cDNAs. Points represent means (± SE) of 7 oocytes (2 donors). AcCho (100 μM) applied at −100 mV as in A. Peak currents normalized to 5.1 μA (mutα7) or to 1.9 μA (mutα7-GFP).