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. 2002 Mar 12;99(6):3950–3955. doi: 10.1073/pnas.052699299

Figure 5.

Figure 5

α-BuTX binding activity in oocytes injected with either mutα7 or mutα7-GFP subunit cDNAs. Columns indicate α-BuTX binding to untreated oocytes (black columns) or oocytes pretreated for 90 min with 1 mM AcCho (open columns). MLA effectively blocked the binding of α-BuTX to both receptors (striped columns). Each column represents data from 27 oocytes (one experiment of two). The oocytes, tested for AcCho sensitivity (100 μM; −100 mV) before the binding experiment, generated AcCho-current amplitudes ranging from 1.5 to 2.3 μA (mutα7 oocytes) and 0.3 to 2 μA (mutα7-GFP oocytes). (−), noninjected oocytes.