Figure 6.

Confocal microscope fluorescent images of oocytes expressing α7-GFP receptors. (A) An oocyte expressing mutα7-GFP. Notice that fluorescence is strongly polarized. Z-scans of animal hemisphere sections: wtα7-GFP (B), mutα7-GFP (C). Surface scans of the animal hemispheres of oocytes expressing wtα7-GFP (D) or mutα7-GFP (E). Surface scan of the vegetal hemisphere of an oocyte expressing wtα7-GFP (F). G and H show a detail (×8 magnification) of the fluorescent clusters sparsely distributed in the vegetal hemisphere of the same oocyte. [Bars = 200 μm (in A ), 5 μm (in B and C), and 20 μm (in D–F).]