Figure 5.

Immunohistochemistry of a reeler mouse brain 4 weeks after HNSC transplantation. (a) Low magnification (×100) of the cortex stained with anti-βIII-tubulin antibody and counterstained with DAPI to reveal nuclei. Note that βIII-tubulin-positive (green) cells are virtually undetectable. (b) Groups of BrdUrd-positive cells near the injection site (×400). Note that the morphology of these cells differs from that of cells expressed in the cortex of wild-type mice (see Fig. 4). (c) Chain migration of transplanted cells expressing reelin (green) in the cortex (×400). (d) Few GFAP-positive HNSCs (brown) detected in the cortex (×100). (e) Differentiation of HNSCs into βIII-tubulin-positive cells (green) near the injection site (×400). Nuclei were counterstained with DAPI (blue).