Fig. 3.

Effect of polyamine depletion on TNFα-stimulated NF-κB activation in C-28/I2 chondrocytes. (A) Cells were grown for 3 days after seeding without any addition (Control), or in the presence of 4 mM DFMO, or in the presence of 4 mM DFMO plus 100 μM putrescine, and then treated with TNFα for 30 min. Cells were collected and nuclear extracts were analyzed for p65 NF-κB DNA binding activity. Data are means ± SEM (N=3); * p<0.05 vs. TNFα-treated cells. (B) The cells, treated as indicated in the legend of panel A, were collected and analysed for the amount of nuclear p65 NF-κB or the level of phosphorylated p65 NF-κB by Western blot.