Fig. 5.
Effect of polyamine depletion on TNFα-stimulated IL-8 production by C-28/I2 chondrocytes. Cells were grown for 3 days after seeding without any addition (Control), or in the presence of 4 mM DFMO, or in the presence of 4 mM DFMO plus 100 μM putrescine, and then treated with TNFα for 24 h. Cells were analyzed for IL-8 content in cell lysates (A) or in cell medium (B). Data are means ± SEM (N=3 for A and N=5 for B); * p<0.05 vs. TNFα-treated cells.