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. Author manuscript; available in PMC: 2006 Sep 1.
Published in final edited form as: J Cell Physiol. 2005 Sep;204(3):956–963. doi: 10.1002/jcp.20368

Fig. 6.

Fig. 6

Effect of TNFα and polyamine depletion on caspase activity and cell viability of C-28/I2 chondrocytes. Cells were grown for 3 days after seeding without any addition (Control), or in the presence of 4 mM DFMO, or in the presence of 4 mM DFMO plus 100 μM putrescine, and then treated with TNFα. After 20h incubation cells were collected and assayed for caspase activity (A). Alternatively, cells viability (B) was evaluated by trypan blue exclusion at the end of a 24h incubation with TNFα. Data are means ± SEM (N=3 for A and N=6 for B); * p<0.05 vs. control cells.