Table 2.
Information of antibodies used in Western blot.
| Antibody name | Host | Dilution | Catalog No. | Manufacturer |
|---|---|---|---|---|
| Anti-LC3 | Rabbit | 1:2500 | 14600-1-AP | Proteintech |
| Anti-ATG5 | Rabbit | 1:2500 | 10181-2-AP | Proteintech |
| Anti-Beclin1 | Rabbit | 1:5000 | 11306-1-AP | Proteintech |
| Anti-p62/SQSTM1 | Rabbit | 1:20000 | 18420-1-AP | Proteintech |
| Anti-PI3K | Rabbit | 1:500 | 20584-1-AP | Proteintech |
| Anti-AKT | Rabbit | 1:5000 | 10176-2-AP | Proteintech |
| Anti-mTOR | Rabbit | 1:5000 | 28273-1-AP | Proteintech |
| Anti-PI3K p85 beta (phospho Y464) | Rabbit | 1:1000 | ab138364 | Abcam |
| Anti-Phospho-AKT (Ser473) | Rabbit | 1:2500 | 28731-1-AP | Proteintech |
| Anti-Phospho-mTOR (Ser2481) | Rabbit | 1:500 | 28879-1-AP | Proteintech |
| Anti-β-actin | Rabbit | 1:10000 | 20536-1-AP | Proteintech |
| HRP-goat anti-rabbit | Goat | 1:10000 | RGAR001 | Proteintech |