Table 1.

Changes in the phosphorylation of Akt and eNOS during stimulated penile erection

Electrical stimulation	% Phospho-Akt-S473	% Phospho-eNOS-S1177
None	100 (4)	100 (6)
1 min	176 ± 33* (3)	164  ± 22* (6)
1 min +  wortmannin	115 ± 7 (4)	159  ± 14 (4)
1 min + LY294002	106 ± 7 (4)	123  ± 13 (4)
Papaverine
None	100 (4)	100 (9)
Papaverine alone	135 ± 7 (4)	171  ± 19* (9)
Papaverine +  wortmannin	86 ± 6 (4)	127  ± 15 (4)
Papaverine +   LY294002	80  ± 17	139  ± 24 (4)

The numbers represent the mean change ± SE from unstimulated (sham) control for the ratio of phosphorylated to total protein as determined by densitometric analysis of Western blots using specific antibodies as described for Fig. 1. The numbers in parentheses indicate measurements per condition. 

^*, P < 0.05 compared to unstimulated condition by t test.