EPR spectra of Chl generated in PSII RC preparations from WT (solid line), D1-H118Q (dashed line), and D2-H117N (dotted line). Instrument conditions: measuring temperature, 4.7 K; microwave frequency, 9.48 GHz; modulation amplitude, 0.2 mT; power, 10 μW; and modulation frequency, 100 kHz.