Fig. 6: Histone modification proteomics using organoids derived from H and H53wt/fl, H53wt/172, H53 fl/fl, and H53 fl/172 mice tumors.
(A) PCA analysis confirms transgene-based lineage relationship and genetic heterogeneity in histone samples. (B) Heatmap showing the top 25 histone modifications of DEGs (abs(log2 FC) > 1, Padj < 0.05) between H, H53 co-mutated organoid samples. (C) Venn diagram shows the shared histone modification marks among H53 co-mutated organoid samples compared with H-only groups. (D) The histone modification identified peaks in the mass spectrometry system. (E) Western blotting results detecting the H3K9ac, H3, TOP1, p-HER2, HER2, KAP1, GAPDH, γ H2Ax, p53, and p73 level in H, H53 co-mutated organoid samples. (F) The model shows histone acetylation in chromatin remodeling by neutralizing the histone charge.