Table 1. Validation of CT/NG detection with the Alinity m STI assay on 13 pooled specimens.

CN: cycle number; Ct: cycle threshold; CT: Chlamydia trachomatis; CV: variation coefficient; ID: identification; NG: Neisseria gonorrhoeae; SD: standard deviation.

^a The variation coefficient (CV) percentage was calculated using the formula: SD/average * 100.

Sample ID	Test on individual specimens				Test on mixed specimens			CN comparison
	Original specimen	Positive target	Ct m2000	CN Alinity m	Specimen(s) pooled in the original specimen	Positive target	CN Alinity m	Difference	Average	SD	CV% a
28731304	Urine	CT	6.22	30.68	Rectal swab	CT	35.59	-4.91	33.14	3.47	10.48
28711078	Urine	CT	12.05	30.60	Oropharyngeal swab	CT	30.25	0.35	30.43	0.25	0.81
28697432	Urine	CT	2.25	30.80	Oropharyngeal swab. Rectal swab	CT	35.85	-5.05	33.33	3.57	10.72
28719466	Urine	CT	6.95	31.37	Rectal swab	CT	33.01	-1.64	32.19	1.16	3.60
28702905	Urine	CT	9.81	31.14	Rectal swab	CT	31.42	-0.28	31.28	0.20	0.63
28747506	Urine	CT	1.57	31.24	Rectal swab	CT	36.20	-4.96	33.72	3.51	10.40
28753798	Urine	CT	7.61	29.04	Rectal swab	CT	32.06	-3.02	30.55	2.14	6.99
28792751	Urine	CT	6,73	30.76	Oropharyngeal swab. Rectal swab	CT	36.59	-5.83	33.68	4.12	12.24
28736005	Rectal swab	CT	10.47	31.47	Urine	CT	26.15	5.32	28.81	3.76	13.06
28713869	Rectal swab	CT	1.98	33.49	Urine	CT	32.67	0.82	33.08	0.58	1.75
28755527	Urine	NG	17.66	20.32	Rectal swab	NG	21,99	-1,67	21,16	1,18	5,58
28754636	Urine	NG	5.15	31.40	Rectal swab	NG	28.92	2.48	30.16	1.75	5.81
28713868	Oropharyngeal swab	NG	0.43	35.18	Rectal swab	NG	35.00	0.18	35.09	0.13	0.36