Fig. 4.

Increased oncogenic phenotypes. A) Logistic model of cell growth after confluence. B) Fitted asymptote from the logistic cell model comparison. C) Representative images of soft agar colonies formed in a single well. D) Summary of soft agar colony counts. E) Representative images of HEK293 transwell migration. F) Quantification of transwell-migrated HEK293 cells. G) Representative images of HEK293 transwell invasion. H) Quantification of transwell invaded HEK293 cells. I) Linear regression of log-wise tumor size to days. Both treatments (c2 and n) are normalized to the first point at which all mice develop tumors. Gray-shaded areas represent error bands indicating the 95 % confidence interval. J) Comparison of fitted slope from linear regression. P-value was derived from the linear mixed effects model for the effect of treatment. K) Mice weight change across days. L) Heteroplasmy of single-cell-derived soft agar colonies does not decay 2 weeks after seeding. All figures with error bars are mean±sem.