Table 2.   Primers used for the qPCR of trace-amine-associated receptors (TAARs) and olfactory signaling pathway genes.

Target gene	Abbreviation	Accession no.	Primer forward	Primer reverese	Product size (bp)
Trace amine associated receptor 1	TAAR1	XM_040667842.2	AGCCAAAAGGCAGGCAAGA	AAGAAGAATGGGCTCCAGCA	153
Trace amine associated receptor 2	TAAR2	XM_040667703.1	TAGGTCACCCGGAGTACGAG	CTCCACAGACCTCACCATGC	208
Trace amine associated receptor 5	TAAR5	XM_040668165.1	TGACCCTTTGCTCTACCCCA	GCCACCCCCAGAGCTTATTG	197
Adenylate cyclase 3	ADCY3	XM_040668402.2	CTTCTCTCGCCACGAGCTGTA	ACTGGGGTTCATCCAGGAGT	179
		XM_040698094.2
		XM_040698095.2
		XM_040668401.2
Anoctamin 2	ANO2	XM_046909374.1	AGTCTGAACAGGCAGAAGGC	AAGTGCATGCCACTCATGGA	224
		XM_046909597.1
Cyclic nucleotide gated channel subunit alpha 2	CNGA2	XM_040669765.2	GACCCTGCAAGGGATTGGTAT	GGTGAAGATGAGGGCTAGAGG	134
		XM_040669764.2
		XM_025149974.3
		XM_025149973.3
Cyclic nucleotide gated channel subunit alpha 4	CNGA4	XM_040657765.2	AGTCCAGTGCCCTCAAACTG	CTGCGCCTTAGTGGGATCTT	179
		XM_015280937.4
Cyclic nucleotide gated channel subunit beta 1	CNGB1	XM_040680816.2	ACATGGGTCTACGATGGGGA	GGTTCTGGACTGTTCTGGGG	283
		XM_040645978.2
		XM_015292311.4
		XM_015292310.4
G protein subunit alpha L	GNAL	XM_046911740.1	GAGGATCCTGCACGTCAATGG	CATGCTGACGCTGTCGATTC	337
		XM_046911739.1
		XM_046926427.1
		NM_001396901.1
		NM_001008746.2

Those with more than one accession No. are due to the presence of more than one chicken BioProject or more than one splice variant.