Figure 2.

Expression pattern of Ca_V1.2^Tg in adult brain. (A) Representative confocal images of anti-HA staining of 25-μm sagittal sections of whole brain (4×). Ca_V1.2^Tg expression is restricted to Ca_V1.2^Tg+; Syn^Tg+ brains (A₃), confirming a lack of transgene expression without co-expression of Cre recombinase. (B–D) Representative confocal images (20×) of sagittal sections of the hippocampus taken from control (B), Ca_V1.2^Tg+; Syn^Tg−(C) and Ca_V1.2^Tg+; Syn^Tg+(D) mice immunostained with antibodies specific for DAPI (blue), MAP2 kinase (red), and HA (green). Staining for the HA tag is only observable in hippocampus recovered from the Ca_V1.2^Tg+; Syn^Tg+ mice (D₃) and is particularly abundant in the DG and CA3 regions (40× images below main images). Note in panels (D₁–D₄), a tile is missing in the lower right corner that was lost during image acquisition. DG, dentate gyrus; GCL, granule cell layer; HA, hemagglutinin.