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. 2025 Jun 19;14(6):3359–3372. doi: 10.21037/tcr-2024-2539

Figure 3.

Figure 3

The correlation between the expression of IL-32 and the enrichment of F. nucleatum in tumor tissue. (A) Representative images of FISH staining for F. nucleatum and corresponding IL-32 IHC staining in LSCC tumor tissue. EUB338, AF488-labeled; F. nucleatum, AF594-labeled. (B) Representative images of FISH staining for F. nucleatum and corresponding IL-32 IHC staining in HSCC tumor tissue. Total magnification for IHC and FISH is 630×. (C) IL-32 expression level between F. nucleatum low and high enriched tumor tissue. (D) LNM percentage between low and high F. nucleatum enriched tumor tissue. AOD, average optical density; IHC, immunohistochemistry; FISH, fluorescence in situ hybridization; IL-32, interleukin-32; F. nucleatum, Fusobacterium nucleatum; LSCC, laryngeal squamous cell cancer; HSCC, hypopharyngeal squamous cell cancer; LNM, lymph node metastasis.