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. 2025 Jun 11;301(7):110364. doi: 10.1016/j.jbc.2025.110364

Figure 2.

Figure 2

EXOSC10 is critical for male germ cell meiosis.A, H&E staining of testicular sections from control and Exosc10 cKO mice at P12 and P21. Scale bar represents 100 μm. B, dual-immunofluorescence staining for DDX4 and γH2AX in P12, P17, and P21 control and Exosc10 cKO testes. DNA was stained with Hoechst 33342. Scale bar represents 100 μm. C, quantification of γH2AX-positive cells per tubule in control and Exosc10 cKO mice at P12, P17, and P21. Data are presented as the mean ± SD from three biologically independent experiments; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. D, coexpression of DDX4 and SYCP3 in P12, P17, and P21 control and Exosc10 cKO testes. Scale bar represents 100 μm. E, statistical analysis of SYCP3-positive cells per tubule in control and Exosc10 cKO mice at P12, P17, and P21. Data are presented as mean ± SD from three biologically independent experiments; ∗p < 0.05, ∗∗∗p < 0.001. F, PNA staining of testicular sections from P21 control and Exosc10 cKO mice. DNA was stained with Hoechst 33342. Scale bar represents 100 μm. G, immunoblot analysis of EXOSC10, DMC1, SYCP3, and γH2AX in P16 control and Exosc10 cKO testes, with β-ACTIN as a loading control. H, relative expression levels of EXOSC10, DMC1, SYCP3, and γH2AX. Data are presented as the mean ± SD from three biologically independent experiments. ∗∗p < 0.01, ∗∗∗p < 0.001. Representative data from n = 3 independent biological replicates (A, B, D, and F) with consistent results across conditions. cKO, conditional KO; DDX4, DEAD box helicase 4; PNA, peanut agglutinin; SYCP3, synaptonemal complex protein 3.