Figure 8.

Exosc10 cKO spermatogenic cells exhibit defective rRNA processing.A, schematic representation of the pre-rRNA processing pathway. B, RT–qPCR analysis of the expression levels of indicated rRNA precursors in isolated spermatogenic cells from control and Exosc10 cKO mice. The expression level of genes in control relative to β-actin was set to 1. Data are presented as mean ±SD from three biologically independent replicates. ns, no significance, ∗p < 0.05, ∗∗p < 0.01. C, model depicting the role of EXOSC10 in driving spermatogonial development and meiotic progression in spermatocytes. The absence of EXOSC10 results in defective rRNA processing, which may impair ribosome assembly, disrupt spermatogonial development, and hinder meiotic progression, culminating in disrupted spermatogenesis and male sterility. cKO, conditional KO; qPCR, quantitative PCR.