Table 2.

Oligonucleotide primers used for polymerase chain reaction amplification in this study.

Target gene	Primer	Sequence (5’ ⟶ 3’)	Annealing temperature (°C)	No. of cycles	Product size (bp)	References
invA	invAf	GTGAAATTATCGCCACGTTCGGGCAA	55	35	284	[13]
	invAr	TCATCGCACCGTCAAAGGAACC
aroC	aroCf	CCTGGCACCTCGCGCTATAC	55	34	826	[18, 20]
	aroCr	CCACACACGGATCGTGGCG
dnaN	dnaNf	ATGAAATTTACCGTTGAACGTGA	55	34	833	[18, 19]
	dnaNr	AATTTCTCATTCGAGAGGATTGC
hemD	hemDf	GAAGCGTTAGTGAGCCGTCTGCG	55	34	666	[18, 20]
	hemDr	ATCAGCGACCTTAATATCTTGCCA
hisD	hisDf	GAAACGTTCCATTCCGCGCAGAC	55	34	894	[18, 19]
	hisDr	CTGAACGGTCATCCGTTTCTG
purE	purEf	ATGTCTTCCCGCAATAATCC	55	34	510	[18, 19]
	purEr	TCATAGCGTCCCCCGCGGATC
sucA	sucAf	CGCGCTCAAACAGACCTAC	55	34	643	[18, 20]
	sucAr	GACGTGGAAAATCGGCGCC
thrA	thrAf	GTCACGGTGATCGATCCGGT	55	34	852	[18, 20]
	thrAr	CACGATATTGATATTAGCCCG
16s rRNA	16SF1	TGTTGTGGTTAATAACCGCA	55	35	572	[22]
	16SIII	CACAAATCCATCTCTGGA