Table 1.

PS pacified: CST; mass/shell-filling analysis

	Compound 1	Compound 2	Compound 3	Compound 4
Formula
[Dc-N-Y]-amide-{Ds-E-Z}n†	[Dc4Y]-amide-{Ds-2.5Z*}n	[Dc5Y]-amide-{Ds-2.5Z**}n	[Dc6Y]-amide-{Ds-3.5Z**}n	[Dc7Y]-amide-{Ds-4.5Z**}n
MW (calculated) for saturated shell‡	90,214	132,461	456,066	894,795
Number of shell tectons observed§/ theoretical¶	4/9	8–10/15	6–8/15	6/27
% Shell filling	44	53–66	40–53	40
MALDI-TOF-MS (MW)	56,496	120,026	227,606	288,970
PAGE (MW)	58,000	116,000	233,000	467,000
AFM observed dimensions	25 × 0.38 nm (D,H)	33 × 0.53 nm (D,H)	38 × 0.63 nm (D,H)	43 × 1.1 nm (D,H)
Calc (MW)‖	56,000	136,000	214,000	479,000

N, nucleophilic dendrimer core reagent; E, electrophilic dendrimer shell reagent; Z, surface group functionality; Z*, EA amide groups; Z**, tris-hydroxymethyl aminomethane amide groups. 

^† In the notation [D_c-N-Y]-amide-{D_s-E-Z}_n above, n = number of dendrimer shell molecules {D_c-E-Z} surrounding the dendrimer core molecule [D_c-N-Y], where [D_c-N-Y] represents amine-terminated, [EDA], generation N, PAMAM dendrimer core reagent and {D_c-E-Z} represents carboxymethyl ester terminated, [EDA], generation E, PAMAM dendrimer shell reagent. 

^‡ MW of theoretical number of shell dendrimer tectons plus core tecton, as determined from MALDI-TOF-MS data for individual core and shell tecton MWs. Values used for n in these calculations were obtained by using the Mansfield–Tomalia–Rakesh equation as described (48, 50). 

^§ Based on the experimental MALDI-TOF-MS MWs of the tecto(dendrimer)s as isolated. 

^¶ The theoretical number (n) of dendrimer shell molecules that would be expected to surround a specific dendrimer core molecule according to the Mansfield–Tomalia–Rakesh equation (48, 50). 

^‖ Calculated MWs based on dome dimensions observed on a mica surface (46).