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. 2002 Apr 16;99(8):5150–5154. doi: 10.1073/pnas.052023199

Figure 4.

Figure 4

Cobalt binding to the Aa,Ab,B2 tetramer. (Upper) Spectrum of Co(II) bound to the Aa,Ab,B2 tetramer. The concentration of the peptides was 40 μM in tetramer (80 μM in metal-binding sites). The extinction coefficient at 550 nm was 140 M−1⋅cm−1 (per tetramer). (Lower) Titration of the Aa,Ab,B2 tetramer with CoCl2. The concentration of peptides was 40 μM in tetramer (80 μM in metal-binding sites). The curve saturates at between 80 and 100 μM, indicating that two cobalt ions are bound per tetramer.