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. 2002 Apr 16;99(8):5195–5200. doi: 10.1073/pnas.032089399

Figure 5.

Figure 5

Inhibition of Fe mineralization in Cr(TREN)–ferritin complexes. Mineralization as a function of [Cr(TREN)] bound per protein subunit is shown. Ferritins (2 μM) were preincubated with Cr(TREN) (0–2.5 mM) for 24 h at 25°C, Fe(II) was added {500 Fe(II) per protein, [Fe] = 1 mM}, and after 2 h incubation, unmineralized Fe was separated from the protein by electrophoresis in native polyacrylamide gels (6%). Mineralization was monitored as the density of Prussian blue formed in protein bands after soaking acidic solutions of potassium ferrocyanide. For each protein, the amount of iron mineralization is shown relative to the iron cores formed in the absence of Cr(TREN). The average of multiple experiments is shown; there was little variation observed in replicate experiments. The number of independent measurements for each type of protein is indicated, with the number of independent protein preparations shown in parenthesis: rH = 3 (1), rH L134P = 4 (3), HoSF = 4 (2), rL = 2 (2), E2 = 2 (1).