Table 1.
Filament polymorphism of fusion proteins compared to Ure2p
Protein | Type* | Diameter,† nm | Repeat length, nm | Frequency,¶ % |
---|---|---|---|---|
Ure21–65-barnase | B | 26 ± 2 | 180 ± 20 | 100 |
Ure21–80-GFP | A | 19 ± 2 | 50–200‡ | 88 |
B | 32 ± 2 | 60–200‡ | 12 | |
Ure21–65-CA | A | 18 ± 2 | 40–60‡ | 87 |
B | 38 ± 2 | 40–300‡ | 13 | |
Ure21–65-GST | A | 24 ± 5 | Not detected | 100 |
Ure2p | — | 30 ± 5 | 55 ± 10§ | — |
Type description: A = apparent single filament; B = bundle of tightly associated type A filaments.
Errors given are one standard deviation based on at least 20 measurements.
For some of the filaments no repeat was detected.
Rare event: for most filaments no repeat could be detected.
Frequencies can vary substantially between different preparations. The values given here are averages for all preparations done. In each case more than 100 filaments were counted.