Figure 5.

Representation bias assessed by competitive genome hybridization to metaphase chromosomes. Amplification reactions included a heat denaturation step, and amplification was carried out as described. Amplified (100,000-fold) and unamplified DNA samples were nick-translated to incorporate biotin nucleotide and digoxigenin nucleotide, respectively. The probes were mixed in equimolar amounts of nucleotide and hybridized without CotI suppression. Specific signals were detected by avidin FITC (amplified DNA, green) and antidigoxigenin rhodamine (unamplified DNA, red).