Figure 3.
Kinetics of tube growth. Typical length (L) vs. time (t) and corresponding instantaneous velocity (V) vs. time (t) plots, for, respectively: (a and b) a free bead in a standard motility assay performed under the same conditions (L corresponding to the distance covered by the bead); (c and d) an EPC tube in phase I; (e and f) an EPC tube in phase II. (g) Average velocities: free bead (Bd), EPC lipid tubes in phases I and II (EPC), Golgi-purified lipid tubes (GPL) in phases I and II, biotinylated purified Golgi membranes in phase II (Gol), and EPC lipids with the addition of 50% (no./no.) cholesterol (Chl) in phase II.