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. 2002 Apr 16;99(8):5436–5441. doi: 10.1073/pnas.062059999

Figure 5.

Figure 5

Loss of BFA1 enhances the interaction between Tem1 and Cdc15. To determine the effect of Bfa1 or Bub2 in the interaction between Cdc15-HA and Tem1-myc, Tem1-myc was immunoprecipitated from S15 fractions prepared from various strains as described in Fig. 3. Cdc15-HA and Tem1-myc proteins were detected with anti-HA and anti-myc antibodies, respectively. Asterisk denotes a cross-reacting protein with an anti-HA antibody. Lanes: 1, strain KLY2170 (TEM1-myc); 2, strain KLY2612 (CDC15-HA); 3, strain KLY2614 (TEM1-myc CDC15-HA); 4, strain KLY2623 (TEM1-myc CDC15-HA bub2Δ); 5, strain KLY2625 (TEM1-myc CDC15-HA bfa1Δ); 6, strain KLY2627 (TEM1-myc CDC15-HA bub2Δ bfa1Δ).