Skip to main content
. 2025 Jun 12;85(12):1121–1133. doi: 10.1002/pros.24925

Figure 5.

Figure 5

Slight changes of gene and protein expression associated with epithelial‐to‐mesenchymal transition (EMT) after knockdown of non‐POU domain‐containing octamer binding (NONO) in prostate cancer cells. (A) Differences in expression of EMT‐associated genes after siRNA transfection in PC3 cells targeting NONO were analyzed by qRT‐PCR and RT2 Profiler PCR array for human EMT genes (for ITGA5, RAC1, and TGFB3). mRNA levels were determined by normalization to the mean of TBP, HPRT, and β‐actin or the mean of ACTB, B2M, GAPDH, HPRT1, and RPLP0 (for ITGA5, RACK1, and TGFB3) and presented as mean fold change ± 95% CI to siControl (for JAG1 and NOTCH1 n = 6; for FGFBP1, GLI1, GNG11, MMP3, SPP1, and TSPAN13 n = 5; for HHIP n = 4; for ITGA5, RAC1, and TGFB3 n = 3). (B) Reduced protein level of NOTCH1 and SNAI1 after knockdown of NONO in PC3 cells was analyzed using Western blotting. β‐Actin was used as a loading control (n = 6). * = p < 0.05, ** = p < 0.01, *** = p < 0.001.