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. 2025 Jul 21;15:26442. doi: 10.1038/s41598-025-10103-0

Table 3.

Summary table of previous studies investigating medieval diets through multi-analytical approaches applied to medieval dental calculus, bone, and ceramic vessels.

Reference Sites Chronology Location Analysis * N. Individuals Main Results
This study Früebergstr.- Baar  7th century CE Switzerland 1. Shotgun proteomics 28

- plant proteins (cereals and Pentapetalae)

- dairy products

- fluvial resources (P. fluviatilis)

45 Dalheim 9th − 12th centuries CE Nord Rhein-Westphalia, Germany

1.aDNA (targeted and shotgun)

2.Proteomics

3.TLM

4. Isotopes

4

- starch grains of Fabaceae/Triticeae

- animal collagen fibers

- sheep, crucifer, pig or boar (genetic data)

- C3 plants and terrestrial animal resources

61 Dalheim 9th − 12th centuries CE Nord Rhein-Westphalia, Germany 1. Stable isotope analysis (bone, dentin) (C, N) 24

- terrestrial resources; C3 plants

- minor consumption of C4 plants

This study Dalheim 9th − 12th centuries CE Nord Rhein-Westphalia, Germany

1. Stable isotope analysis (bone, dentin) (C, N)

2. Shotgun proteomics

8 (isotopes)

24 (proteomics)

- plant proteins (cereals, legumes, e.g. P. sativum, Pentapetalae, Chenopodiaceae)

- fluvial resources (P. fluviatilis)

78

Altenerding/

Straubing-Bajuwarenstrasse

5th − 7th centuries CE Bavaria, Germany 1. Stable isotope analysis (bone) (C, N) 179

- C3 plants

- low animal products

79 8 sites 5th − 7th centuries CE Bavaria, Germany - Stable isotope analysis (bone, dentin) (C, N)

102 (bone)

24 (dentin)

- C3 plants

- low intake of meats

- low amounts of freshwater fish

4 9 sites 6th −  11th centuries CE South Tyrol, Italy 1. Stable isotope analysis (bone) (C, N, S) 91

- C3 plants

- C4 plants

- terrestrial animal products

46 Santa Severa 5th − 7th   CE Lazio, Italy

1. Stable isotope analysis

2. TLM (calculus) and

3. GC–MS (calculus)

4. aDNA (calculus)

−120

−94

- C3-terrestrial protein; minor

- C4 plant consumption

- metabolites herbs and wine

- medicinal plants (e.g., Punica granatum, Ephedra sp.)

80 Mikulčice 9th − 10th centuries CE Czech Republic - Stable isotope analysis (dentin, bone) 92

- C3 plants

- C4 plants

- meats

81 San Pietro 7th −  16th centuries CE Veneto, Italy

1. Stable isotope analysis (bone)

2. TLM (calculus)

6

- C3 plants

- C4 plants

- terrestrial animal products

28 14 sites 8th century BC − 19th century CE UK 1. Shotgun proteomics (calculus) 100

- dairy products

- plant proteins (Avena sativa, Pisum sativum)

- one animal protein, i.e., haemoglobin (Pecora)

43 Colonna 8th −  10 th centuries CE Lazio, Italy

1. TLM (calculus)

2. GC–MS (calculus)

47

- microremains of both C3 and C4 plants

- chromatographic data: aquatic resources, wine, tea, medicinal plants

35 Tjaerby 12th − 16th centuries CE Denmark 1. Shotgun proteomics (calculus) 21

- dairy products

- plant proteins (Avena sativa)

82 Dunes Abbey of Koksijde 12th − 15th centuries CE Belgium - Stable isotope analysis (bone) (C, N) 19 - terrestrial meats and marine resources
83 West Cotton 10th −  15 th centuries CE Northamptonshire, UK

1. Stable isotope analysis (pottery)

2. GC–MS (pottery)

123 bits from 73 vessels

- dairy products

- cruciferous vegetables

*TLM: Transmitted Light Microscopy; GC–MS: Gas Chromatography-Mass Spectroscopy; aDNA: ancient DNA.