Table 1.

Clonally expanded mutations detected in single cells

Lane no.	Position, bp	Nucleotide change	Cell type	Age, years	Mutant fraction*, %	SD†, %
1	73	G→A	Buccal	76	39	5
2	189	A→G	Myocyte	109	71	8
3	189	A→G	Myocyte	109	58	5
4	214	A→G	Buccal	85	60	14
5	251	G→C	Buccal	93	98	2
6	252	T→C	Buccal	85	69	23
7	303–310	ins C	Buccal	5	30	11
8	303–310	ins C	Buccal	76	61	3
9	303–310	ins C	Buccal	76	28	3
10	303–310	ins 1,2C	Buccal	76	83	4
11	303–310	ins 1,2C	Buccal	76	43	4
12	303–309	ins 2,3,4C	Buccal	85	100	0
13	303–309	ins C	Buccal	85	43	15
14	303–310	del C	Buccal	93	95	7
15	303–310	ins C	Buccal	93	29	1
16	303–310	del C	Buccal	93	88	1
17	303–310	ins 1,2C	Buccal	93	53	9
18	303–310	ins 1,2,3C	Buccal	93	94	1
19	303–310	del C	Myocyte	104	28	3
20	16,028	T→C	Myocyte	51	65	7
21	16,029	T→C	Myocyte	109	53	4
22	16,029	T→C	Myocyte	109	57	4
23	16,033	G→A	Myocyte	109	50	14
24	16,034	G→A	Myocyte	109	25	1
25	16,035	G→A	Myocyte	104	58	4
26	16,035	G→A	Myocyte	109	100	0
27	16,036	G→A	Myocyte	104	73	10
28	16,036	G→A	Myocyte	109	91	8
29	16,049	G→A	Myocyte	109	80	8
30	16,052–3	del C	Myocyte	104	78	11
31	16,054	A→G	Myocyte	51	65	1

Note that generally there was one mutation per cell except for the four cells in which there were two mutations (mutations in lanes 1 and 10, 5 and 15, 2 and 21, and 3 and 29). 

^*The averages are of at least two independent measurements from two independent PCR amplifications from the same cell. 

^† Formal standard deviations are given to illustrate the extent of reproducibility of the data.