Table 2.

EAE incidence and progression for CD8⁺ −/− and +/+ on 0.87% Ca²⁺ diets with or without 100 ng of 1,25-(OH)₂D₃ per day

	Incidence	Mean severity	Mean day of onset	Serum calcium, mg %
CD8+ +/+	62.5% (n = 4 m, 4 f)	2.0  ± 0	20  ± 4	10.0  ± 0.6
CD8+ −/−	87.5% (n = 4 m, 4 f)	2.9  ± 1.1	18  ± 4	10.6  ± 0.8
CD8+ +/+, + 1,25 D3	0%* (n = 3 m, 4 f)	—	—	14.1  ± 0.8†−
CD8+ −/−, + 1,25 D3	0%† (n = 4 m, 3 f)	—	—	12.8  ± 1.3†−

CD8⁺ −/− and +/+ mice were switched at 5 weeks of age to a 0.87% Ca²⁺-purified diet containing 0 ng/day or 100 ng/day of 1,25-(OH)₂D₃. Ten days later, 400 μg of MBP was injected s.c. along with an i.p. injection of 200 ng of pertussis. A second pertussis toxin injection was given 48 h later. Mice were scored for EAE once per day for 40 days. For mice that developed an EAE score of 2 or greater, the incidence, mean peak severity ± SD, and mean day of onset ± SD were calculated. Values adjacent to incidence rates indicate the number of male and female mice in the group. At the end of study, mice were killed and serum calcium concentrations determined by atomic spectrometry. (*, P < 0.05;  

^†, P < 0.005)