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. 2002 Apr 16;99(8):5616–5621. doi: 10.1073/pnas.082613599

Figure 6.

Figure 6

Rescue of utrophin, dystroglycan, sarcoglycan, and dystrobrevin expression in mdx/CT mice. Immunoblotting of 20 μg of muscle cell protein from 5-week-old wild-type (Wt), transgenic (CT), mdx, and mdx/CT animals showed that mdx/CT mice express levels of α- and β-dystroglycan, utrophin, α, β, and γ, sarcoglycan, and dystrobrevins that are equal to or higher than those found in Wt animals. Expression of all DAPs in mdx/CT were between −14% and + 30% of wild type levels, except for utrophin (+538%) and γ sarcoglycan (+660%), which were increased by very large amounts. Utrophin levels were also dramatically higher at 6 months of age. By contrast, all DAPs were reduced in expression in mdx muscles when compared with wild-type (−36% and −70% for α- and β-dystroglycan, −95%, −30%, and −50% for α, β, and γ sarcoglycan, and −78% for utrophin). Molecular weights shown are ≈400 kDa for utrophin and dystrophin. ≈160 kDa for α-dystroglycan, 43 kDa for β-dystroglycan, 50 kDa for α-sarcoglycan, 43 kDa for β-sarcoglycan, 35kDa for γ-sarcoglycan, 53 kDa for desmin (with 50 kDa lower band in CT and mdx/CT), ≈140–180 kDa for NCAM, and 90, 65, 44, and 22 kDa for dystrobrevin.