Table 1.
Me
|
H2/CO2
|
Me/H2/CO2
|
Ac
|
|||||
---|---|---|---|---|---|---|---|---|
−pyr | +pyr | −pyr | +pyr | −pyr | +pyr | −pyr | +pyr | |
Wild type | + | + | + | + | + | + | + | + |
Δech1∷pac-ori-aph | + | + | − | − | − | + | − | − |
Δech1∷pac-ori-aph hpt∷echABCDEF | + | ND | + | ND | + | ND | + | ND |
Growth was scored in HS broth with the indicated substrates; Me, methanol; Ac, acetate; pyr, sodium pyruvate. Positive cultures (+), except acetate, typically grew within 2 days; Ac cultures grew within 4 weeks. ND, not done.