Table 3.
Reactions catalyzed by cell extracts
| Reaction | M. barkeri (nmol min−1 mg−1) | M. barkeri Ech1 (nmol min−1 mg−1) |
|---|---|---|
| Ferredoxin-dependent hydrogenase | 130 (±10) | ≪1 |
| CHO-MFR dehydrogenase | 1350 (±50) | 1700 (±100) |
| CHO-methanofuran:H4SPT formyltransferase | 6300 (±880) | 5780 (±960) |
| Methenyl-H4SPT cyclohydrolase | 1350 (±210) | 1200 (±100) |
| Methylene-H4SPT dehydrogenase | 3800 (±540) | 3100 (±170) |
| F420-reducing hydrogenase | 3700 (±260) | 4600 (±330) |
Reactions were assayed as described in Materials and Methods. Details are provided in the supporting information.