Table 1.

Antibodies.

	Primary antibody	Dilution			Secondary	Dilution
Immunofluorescence
	MYH type IIa (DSHB, SC-71)	1:600			Alexa Fluor 488 IgG1	1:500
	MYH Type I (DSHB, BA-D5)	1:50			Alexa Fluor 555 IgM	1:500
	MYH Type IIb (DSHB, BF-F3)	1:100			Alexa Fluor 350 IgG2b	1:500
	PAX7 (DSHB, PAX7)	1:10			Alexa Fluor 488 IgG	1:500
	LAM/laminin (Abcam, ab11575)	1:500			Alexa Fluor 568 IgG	1:500
	Antibody	Dilution		Notes
Immunoblotting
	BNIP3L/Nix (Cell Signaling Technology [CST], 12396)	1:1000
	BNIP3 (CST, 3769)	1:1000
	Cre (CST 15,036)	1:1000
	TUBB/β-tubulin (CST 86,298)	1:10000
	SMAD2-SMAD3 (CST, 8685)	1:1000
	Phoshp-SMAD2 (CST 18,338)	1:1000
	MB/Myoglobin (CST 25,919)	1:10000		Reduced Protein (5 µg). Reduced secondary antibody (1:100,000)
	PPARGC1A/PGC1-α (Santa Cruz Biotechnology 13,067)	1:100
	NFE2L2/NRF2 (CST 12,721)	1:1000
	AIFM1/AIF1 (CST, 5318	1:1000
	ATP2A/SERCA CST, 9580)	1:1000
	SQSTM1/p62 (CST, 5114)	1:1000
	LC3A (CST, 4599)	1:1000
	Donkey anti-rabbit HRP (Jackson Immunoresearch, 711-035-152)	1:10000
	Donkey anti-mouse HRP (Jackson Immunoresearch, 715-035-150)	1:10000

DSHB, Developmental Studies Hybridoma Bank; all DSBH antibodies used are purchased as concentrated supernatant.