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Attachment of oligomerized 2-Cys Prx to the thylakoid membrane in intact chloroplasts. Chloroplasts were isolated from Hordeum leaves and incubated in the absence (lanes 1–4) or presence (lanes 5–8) of 10 mM ascorbic acid. After 5 or 60 min, the chloroplasts were lysed by hypoosmotic shock and the thylakoids (T) separated from the soluble stroma (S) by centrifugation. Proteins from both fractions were separated by SDS/PAGE and analyzed in a Western blot analysis with anti-2-Cys Prx.
