Figure 5.

Reduction of heterologously expressed 2-Cys Prx in an enzymic system containing spinach Trx f or m. The complete electron transport chain is also depicted. After incubation for 30 min, the samples were separated by denaturing nonreducing SDS/PAGE and analyzed in a Western blot analysis with anti-2-Cys Prx. Lanes 1–5 represent samples in which each one component of the complete system had been omitted as indicated on top of each lane. Lane 6 depicts the result with Trx m and lane 7 with Trx f. The position of monomeric reduced 2-Cys Prx is shown. The protein band beneath the monomer indicates degradation. It was a repeated observation that the reduced 2-Cys Prx exhibited a higher propensity for degradation.