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. Author manuscript; available in PMC: 2025 Jul 23.
Published in final edited form as: Physiol Rev. 2025 Jan 15;105(3):1291–1428. doi: 10.1152/physrev.00034.2023

Figure 2. Identifying domain maps of NOX membrane and cytosolic regulatory subunits.

Figure 2.

Structural domains within membrane and cytosolic subunits of the NOX isoforms. NOXs 1 – 5 contain requisite 6 transmembrane domains (TM) and cytosolic NADPH and FAD binding domains. NOX5 and DUOXs 1 & 2 possess Ca2+ binding EF hand domains toward their N-terminus; wherein the DUOXs 1 & 2 also contain an extra TM domain with a tethered peroxidase-like domain. A hallmark proline rich domain (PRR) in p22phox, p47phox and NOXO1 is key for its interaction with Src homology domains (SH3) within p47phox, p67phox and NOXA1, respectively. Tetratricopeptide repeats (TPRs) in p67phox permits its binding to Rac1/2 and a Phox and Bem1 (PB1) domain is required for its interaction with p40phox. PX domains in p47phox, p40phox and NOXO1 facilitate binding to phosphoinositides on the plasma membrane. Different domains in Rac1 include the nucleotide binding sites (NBS), switch 1, switch 2, polybasic region (PRB), insert region (IR) and the CAAX box.