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. 2002 Apr 30;99(9):5884–5889. doi: 10.1073/pnas.092669999

Figure 2.

Figure 2

Southern blot of T. brucei procyclic genomic DNA digested with a panel of restriction endonucleases and probed for T. brucei galE. Approximately five micrograms of DNA, digested overnight with BamHI, NcoI, PstI, SacI, and XhoI restriction enzymes, was loaded per lane. Hybridization was at 60°C overnight. The blot was washed with 1× SSC (1× SSC = 0.15 M sodium chloride/0.015 M sodium citrate, pH 7)/0.1% SDS for 20 min at 60°C, followed by 0.5× SSC/0.1% SDS for 20 min at 60°C. The single band in each lane shows that the epimerase is present as a single copy per haploid genome.