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trHbN is required for aerobic metabolism of NO in M. bovis BCG (ATCC 35734). NO (A, 1.0 μM; B, 0.5 μM) was added to buffer or cell suspensions. NO was monitored with an NO electrode. (C) Aliquots of cell suspensions were taken for Western blot analysis of soluble proteins by using the polyclonal antibodies raised against trHbN.
