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Inhibitory effect of APC on CK2 activity. The kinase activities of GST-CK2α (≈0.5 μg) and a 1:1 mixture of GST-CK2α (≈0.5 μg) and GST-CK2β (≈0.5 μg) were measured against substrate peptide in the presence or absence of anti-APC or human IgG immunoprecipitates from TIG-3 or SW480 cells arrested at G2/M with nocodazole. Results were expressed as mean ± SD of three independent experiments.
