Figure 6.

Effect of APC fragments on growth and transformation properties. (A) 293 cells (2 × 10⁴) constitutively expressing an APC fragment 2086–2394 (APC-C) in pEGFP vector (○) or in pCDNA3-FLAG (♦) vs. wild-type cells expressing empty pEGFP (■) were split to 35-mm plates, and cell counts were monitored at each time point. (B) Triplicate plates of SW480 cells (1 × 10⁶) were transfected with 0.3 μg of either pEGFP-APC-C (column 2), pCDNA3-FLAG-APC-C (column 3), or empty pEGFP (column 1), plated in soft agar, and assayed for colony formation after 3 weeks. Results were expressed as means ± SD of three independent experiments.