Table 1.

Summary of FRET (steady-state and lifetime FRET), and LRET measurements

Sample	Steady-state FRET	Lifetime FRET	LRET*
			τda	τad
Cys-73
Without actin
% ET	65.8  ± 0.5%	69.0  ± 6.7%	66.4  ± 8.6%	85.0  ± 1.8%
Distance	48.5  ± 0.2A	47.3  ± 2.4A	50.0  ± 3.3A	42.0  ± 1.0A
	n  = 2	n  = 3	n  = 2	n  = 2
With actin
% ET	59.6  ± 4.3%	67.8  ± 6.2%	59.4  ± 1.7%	86.5  ± 3.3%
Distance	50.7  ± 1.4A	48.4  ± 3.1A	52.6  ± 1.8A	40.7  ± 2.2A
	n  = 2	n  = 2	n  = 2	n  = 2
Cys-94
Without actin
% ET	57.2  ± 9.3%	47.1  ± 0.1%	66.8  ± 2.8%	88.0  ± 2.4%
Distance	54.7  ± 3.3A	56.1  ± 0.04A	47.2  ± 1.0A	38.0  ± 1.5A
	n  = 5	n  = 3	n  = 2	n  = 2
With actin
% ET	59.8  ± 9.5%	43.1  ± 0.2%	69.2  ± 4.5%	86.4  ± 2.8%
Distance	51.1  ± 4.1A	57.6  ± 0.09	46.4  ± 1.7A	39.0  ± 1.6A
	n  = 4	n  = 2	n  = 2	n  = 2
Cys-2
Without actin
% ET	7.5  ± 1.7%	No detectable ET	50.7  ± 9.5%	81.2  ± 1.4%
Distance	75  ± 2A		55.9  ± 3.6A	43.5  ± 1.1A
	n  = 2		n  = 2	n  = 2
With actin
% ET	6.1  ± 0.3%	No detectable ET	57.1  ± 2.3%	76.5  ± 4.9%
Distance	79  ± 1A		53.7  ± 1.0A	46.1  ± 2.3A
	n  = 2		n  = 2	n  = 2

The energy transfer (ET) and distance from residues 73 to 73, 94 to 94, and 2 to 2 are shown with and without actin. 

^*τ_da indicates donor lifetime in the presence of acceptor. τ_ad indicates sensitized emission lifetime (see FRET, LRET, and Anisotropy Measurements.