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Schematics of internal poly(A) priming by oligo(dT) primer. Besides priming the poly(A) sequence at the 3′ end of mRNA, the oligo(dT) primer can also prime the poly(A) sequences present internally within mRNA. The initiation of cDNA synthesis from the oligo(dT) primed at both locations results in the generation of two truncated cDNAs from a single mRNA template.
