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Comparison of internal poly(A) priming and 3′ poly(A) priming between oligo(dT) primer and anchored oligo(dT) primers. An in vitro transcript containing an internal A20 and 3′ A50 was used for reverse transcription with either oligo(dT) primer or anchored oligo(dT) primers. (A) Sequencing image of cDNA clones primed by oligo(dT) primer and anchored oligo(dT) primers. (B) Summary of the results of the analyses.
